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map k 10 reference strain  (DNASTAR)


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    DNASTAR map k 10 reference strain
    Map K 10 Reference Strain, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 5819 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/map+k+10+reference+strain/MegAlign/us10151004-161-10-20
    Average 99 stars, based on 5819 article reviews
    map k 10 reference strain - by Bioz Stars, 2026-08
    99/100 stars

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    Figure 3. Performance of the 6 qPCR designs using different amounts of <t>MAP</t> K-10 <t>DNA</t> diluted in dilution buffer (A) or fecal DNA extract (B). A serial dilution of DNA from the MAP K-10 reference strain was used to construct a standard curve including 10, 20, 100, 200, 1,000, 2,000, and 10,000 genome equivalents of MAP. The different qPCR assay designs, namely “Kim,” “Slana,” “Herthnek,” “Donaghy,” “Vary,” and “Bannantine,” were analyzed using MAP DNA either diluted in dilution buffer (pure DNA) or spiked into free-MAP fecal DNA extract (spiked feces). The least squares means of the qPCR Cycle of quantification (Cq) with different letters differ (P < 0.05).
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    Figure 3. Performance of the 6 qPCR designs using different amounts of <t>MAP</t> K-10 <t>DNA</t> diluted in dilution buffer (A) or fecal DNA extract (B). A serial dilution of DNA from the MAP K-10 reference strain was used to construct a standard curve including 10, 20, 100, 200, 1,000, 2,000, and 10,000 genome equivalents of MAP. The different qPCR assay designs, namely “Kim,” “Slana,” “Herthnek,” “Donaghy,” “Vary,” and “Bannantine,” were analyzed using MAP DNA either diluted in dilution buffer (pure DNA) or spiked into free-MAP fecal DNA extract (spiked feces). The least squares means of the qPCR Cycle of quantification (Cq) with different letters differ (P < 0.05).
    Map K 10 Reference Strain, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 3. Performance of the 6 qPCR designs using different amounts of MAP K-10 DNA diluted in dilution buffer (A) or fecal DNA extract (B). A serial dilution of DNA from the MAP K-10 reference strain was used to construct a standard curve including 10, 20, 100, 200, 1,000, 2,000, and 10,000 genome equivalents of MAP. The different qPCR assay designs, namely “Kim,” “Slana,” “Herthnek,” “Donaghy,” “Vary,” and “Bannantine,” were analyzed using MAP DNA either diluted in dilution buffer (pure DNA) or spiked into free-MAP fecal DNA extract (spiked feces). The least squares means of the qPCR Cycle of quantification (Cq) with different letters differ (P < 0.05).

    Journal: Journal of dairy science

    Article Title: Systematic assessment of the reliability of quantitative PCR assays targeting IS900 for the detection of Mycobacterium avium ssp. paratuberculosis presence in animal and environmental samples.

    doi: 10.3168/jds.2023-24566

    Figure Lengend Snippet: Figure 3. Performance of the 6 qPCR designs using different amounts of MAP K-10 DNA diluted in dilution buffer (A) or fecal DNA extract (B). A serial dilution of DNA from the MAP K-10 reference strain was used to construct a standard curve including 10, 20, 100, 200, 1,000, 2,000, and 10,000 genome equivalents of MAP. The different qPCR assay designs, namely “Kim,” “Slana,” “Herthnek,” “Donaghy,” “Vary,” and “Bannantine,” were analyzed using MAP DNA either diluted in dilution buffer (pure DNA) or spiked into free-MAP fecal DNA extract (spiked feces). The least squares means of the qPCR Cycle of quantification (Cq) with different letters differ (P < 0.05).

    Article Snippet: Positive control reactions containing 100 genome copies of the MAP K-10 reference strain DNA (ATCC BAA-968) was used to assess plate-to-plate (run-to-run) variability.

    Techniques: Serial Dilution, Construct

    Figure 4. Efficiency of qPCR reaction (%Eff) of the 6 qPCR de- signs, namely “Kim,” “Slana,” “Herthnek,” “Donaghy,” “Vary,” and “Bannantine.” Each %Eff was calculated from the slope of a standard curve produced using MAP DNA either diluted in dilution buffer (pure DNA) or spiked into free-MAP fecal DNA extract (spiked feces). The least squares means with different letters differ (P < 0.05). The asterisks report a matrix effect (P < 0.05).

    Journal: Journal of dairy science

    Article Title: Systematic assessment of the reliability of quantitative PCR assays targeting IS900 for the detection of Mycobacterium avium ssp. paratuberculosis presence in animal and environmental samples.

    doi: 10.3168/jds.2023-24566

    Figure Lengend Snippet: Figure 4. Efficiency of qPCR reaction (%Eff) of the 6 qPCR de- signs, namely “Kim,” “Slana,” “Herthnek,” “Donaghy,” “Vary,” and “Bannantine.” Each %Eff was calculated from the slope of a standard curve produced using MAP DNA either diluted in dilution buffer (pure DNA) or spiked into free-MAP fecal DNA extract (spiked feces). The least squares means with different letters differ (P < 0.05). The asterisks report a matrix effect (P < 0.05).

    Article Snippet: Positive control reactions containing 100 genome copies of the MAP K-10 reference strain DNA (ATCC BAA-968) was used to assess plate-to-plate (run-to-run) variability.

    Techniques: Produced